EC Number   |
|---|
   3.4.24.26 | - |
| 3.4.24.26 | native enzyme 2.23fold to homogeneity by ultrafiltration, gel filtration, and anion exchange chromatography |
| 3.4.24.26 | native extracellular enzyme from cell culture medium by ammonium sulfate fractionation, dialysis, anion exchange chromatography, and cation exchange chromatography |
| 3.4.24.26 | native extracellular enzyme from culture supernatant by anion exchange chromatography and ultrafiltration |
| 3.4.24.26 | recombinant wild-type and mutant enzymes from Escherichia coli strain BL21 (DE3) by ammonium sulfate fractionation, anion exchange chromatography |
| 3.4.24.26 | solubilized recombinant His-tagged enzyme from Escherichia coli cells by metal affinity chromatography, or the enzyme is simultaneously further purified, refolded and buffer-exchanged on a preparative Superdex 200 column by a modified urea reverse-gradient gel filtration |
| 3.4.24.26 | the propeptide-elastase complex |
| 3.4.24.26 | to homogeneity |
