3.4.23.36 | native enzyme from inner membrane to homogeneity by solubilization from membrane with Triton X-100, heat treatmemt at 65°C and pH 4.0, anion exchange chromatography, and chromatofocusing, 35000fold, recombinant enzyme by ammonium sulfate fractionation, anion exchange chromatography in presence of EDTA, and gel filtration to homogeneity |