EC Number   |
|---|
   3.4.22.B6 | - |
| 3.4.22.B6 | ammonium sulfate precipitation, immobilized metal ion affinity chromatography (Ni2+) |
| 3.4.22.B6 | cation-exchange chromatography (Mono S) |
| 3.4.22.B6 | cystatin affinity chromatography |
| 3.4.22.B6 | from supernatant, immobilized metal ion affinity chromatography (Ni2+), ion-exchange chromatography (Mono-Q) |
| 3.4.22.B6 | immobilized metal ion affinity chromatography (Ni2+) |
| 3.4.22.B6 | recombinant GST-tagged enzyme from Escherichia coli strain BL21 to homogeneity, the tag is cleaved off by thrombin |
| 3.4.22.B6 | recombinant His6-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and dialysis |
| 3.4.22.B6 | recombinant prrotein |
