EC Number   |
|---|
   3.4.22.B50 | by His-Bind Purification Kit |
| 3.4.22.B50 | gel filtration |
| 3.4.22.B50 | GST resin column chromatography, Mono Q column chromatography, and Superdex 75 gel filtration |
| 3.4.22.B50 | Ni-NTA column chromatography |
| 3.4.22.B50 | Ni-NTA column chromatography, Superdex 75 gel filtration, and Source 15Q column chromatography |
| 3.4.22.B50 | purified to homogeneity by hydrophobic interaction and anion exchange chromatography |
| 3.4.22.B50 | recombinant His6-tagged wild-type and mutant enzymes from Escherichia coli strain BL21(DE3), the secretion tag is removed and the purification continued by nickel affinity chromatography, gel filtration, and ultrafiltration |
