EC Number |
---|
3.4.22.48 | - |
3.4.22.48 | cysteine protease StpA2aur CH-91 purified by gel filtration |
3.4.22.48 | from culture supernatant, apparant homogenity |
3.4.22.48 | His-Select nickel affinity column chromatography |
3.4.22.48 | ion-exchange chromatography, gelfiltration, immobilized metal ion affinity chromatography for His-tagged protein |
3.4.22.48 | native from culture supernatant |
3.4.22.48 | purified by gel filtration |
3.4.22.48 | recombinant and native protein, gel filtration |
3.4.22.48 | recombinant co-expressed His-tagged staphopain A and inhibitor staphostatin A from Escherichia coli strain BL21(DE3) inclusion bodies by refolding and nickel affinity chromatography, method optimization |
3.4.22.48 | recombinant GST-fusion prostaphopain B from Escherichia coli strain BL21(DE3) by glutathione affinity chromatography, gel filtration, and cleavage of the GST-tag followed by ultrafiltration |