EC Number   |
|---|
   3.4.22.33 | - |
| 3.4.22.33 | 2 forms: FBA and FBB |
| 3.4.22.33 | bromelain from pineapple stem and skin are recovered by a PEG 4000/phosphate aqueous two-phase systems (ATPs) liquid-liquid extraction |
| 3.4.22.33 | CM-Sepharose column chromatography, SP-Sepharose column chromatography, and Sephadex G-50 gel filtration |
| 3.4.22.33 | fractionation |
| 3.4.22.33 | fruit bromelain A, B and C |
| 3.4.22.33 | high-speed counter-current chromatography coupled with the reverse micelle solvent system consisting of 0.10 g/ml cetyltrimethylammonium bromide (CTAB)/isooctane-hexylalcohol and 0.05 M sodium phosphate buffer is successfully applied to separate bromelain from pineapple fruit |
| 3.4.22.33 | native enzyme 2.24fold |
| 3.4.22.33 | native enzyme 2.88fold |
| 3.4.22.33 | native enzyme 7.23fold from fruits by ammonium sulfate fractionation, dialysis, and anion exchange chromatography |
