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Search Purification (Commentary)

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EC Number Purification (Commentary)
Display the word mapDisplay the reaction diagram Show all sequences 3.4.15.6-
Display the word mapDisplay the reaction diagram Show all sequences 3.4.15.6centrifugation, proteins are concentrated and desalted using a cross-flow polyethersulfone cassette with a cutoff point of 30 kDa, a purification method for cyanophycinase is developed which depended on the strong affinity of the enzyme in the crude extract for its insoluble substrate cyanophycin
Display the word mapDisplay the reaction diagram Show all sequences 3.4.15.6from culture supernatant of Pseudomonas anguilliseptica cells grown in medium containing cyanophycin granules, anion-exchange chromatography and L-arginine-agarose affinity chromatography. In case of heterologous expression in Escherichia coli the enzyme was not secreted and thus, isolation was done from the cell lysate
Display the word mapDisplay the reaction diagram Show all sequences 3.4.15.6several steps
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