EC Number   |
|---|
    3.3.2.1 | - |
| 3.3.2.1 | Ni2+ chelating Sepharose column chromatography and Superdex 200 gel filtration |
| 3.3.2.1 | purification is obtained by two metal chelate chromatography steps seperated by emoval of the His tag with TEV protease |
| 3.3.2.1 | recombinant N-terminally His6-tagged AngB-ISC encoding residues 1-216 from Escherichia coli strain BL21(DE3) by nickel affinity chromatography, tag cleavage by TEV protease, another step of nickel affinity chromatography, and gel filtration |
| 3.3.2.1 | recombinant N-terminally His6-tagged VabB-ISC encoding residues 1-217 from Escherichia coli strain BL21(DE3) by nickel affinity chromatography, tag cleavage by TEV protease, another step of nickel affinity chromatography, and gel filtration |
