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Search Purification (Commentary)

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EC Number Purification (Commentary)
Display the word mapDisplay the reaction diagram Show all sequences 3.2.1.84-
Display the word mapDisplay the reaction diagram Show all sequences 3.2.1.842-fold purification and 98% residual enzyme activity after precipitation with propanol, around 2-fold purification and 77% enzyme recovery after salting out with ammonium sulfate at 50% saturation, 10-fold concentrated preparation of the enzyme with a yield of 98% after ultrafiltration, mutanase recovery of 97% after lyophilization and concentration of the culture broth in a vacuum evaporator
Display the word mapDisplay the reaction diagram Show all sequences 3.2.1.84by chromatographic procedures
Display the word mapDisplay the reaction diagram Show all sequences 3.2.1.84native extracellular enzyme 18.4fold by ultrafiltration, anion exchange and hydrophobic interaction chromatography, and chromatofocusing to homogeneity
Display the word mapDisplay the reaction diagram Show all sequences 3.2.1.84yield of 56.3% of alkali-soluble and water-insoluble D-glucan
Results 1 - 5 of 5