EC Number |
---|
3.2.1.70 | - |
3.2.1.70 | by Ni-chelating column chromatography |
3.2.1.70 | by nickel-chelating chromatography with Chelating Sepharose FF |
3.2.1.70 | Ni-chelating column chromatography and Superdex 200 gel filtration |
3.2.1.70 | Ni-NTA column chromatography |
3.2.1.70 | partially |
3.2.1.70 | recombinant His6-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography, anion exchange chromatography, and ultrafiltration, to homogeneity |
3.2.1.70 | wild-type enzyme and mutant enzymes E458Q and E656Q |