EC Number |
---|
3.2.1.68 | - |
3.2.1.68 | ammonium sulfate precipitation, DEAE cellulose column chromatography, CM-cellulose column chromatography, and DEAE-Sephadex gel filtration |
3.2.1.68 | ammonium sulfate precipitation, Ni-NTA column chromatography, and Superdex 200 gel filtration |
3.2.1.68 | copurification of native isozymes ISA1 and ISA2 partially from leaves by gel filtration, copurification of TAP-tagged ISA1 and ISA2 from Escherichia coli strain BL21(DE3) by tandem-affinity chromatography on TEV and calmodulin, followed by gel filtration |
3.2.1.68 | HiTrap chelating column chromatography |
3.2.1.68 | HiTrapQ column chromatography and Superdex 200 gel filtration |
3.2.1.68 | HitrapQ column chromatography and TSKgel DEAE-5PW column chromatography |
3.2.1.68 | HitrapQ column chromatography and TSKgel DEAE-5PW gel filtration |
3.2.1.68 | native extracellular enzyme 369fold from culture supernatant by ultrafiltration, ammonium sulfate fractionation, gel filtration, two different steps of anion exchange chromatography, and dialysis, to homogeneity |
3.2.1.68 | native subunit ISA2 from leaves by anion exchange chromatography, ultrafiltration, and gel filtration, separation from subunit ISA1 |