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EC Number
Purification (Commentary)
3.2.1.63
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3.2.1.63
Ni-NTA bead chromatography
3.2.1.63
Ni2+ affinity chromatography
3.2.1.63
of the recombinant protein by saturated ammonium sulfate precipitation and Q sepharose fast flow, CHT ceramic hydroxyapatite and gel filtration column chromatography
3.2.1.63
partial
3.2.1.63
recombinant
3.2.1.63
recombinant enzyme from Pichia pastoris by gel filtration and anion exchange chromatography, recombinant His6-tagged and MBP-fused crystallin domain from Escherichia coli by nickel affinity chromatography
3.2.1.63
wild-type and mutant derivatives of the catalytic domain of AfcA (Fuc domain)
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