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Search Purification (Commentary)

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EC Number Purification (Commentary)
Display the word mapDisplay the reaction diagram Show all sequences 3.2.1.167-
Display the word mapDisplay the reaction diagram Show all sequences 3.2.1.167native enzyme 7.2fold from roots, by ammonium sulfate fractionation and anion exchange chromatography to homogeneity
Display the word mapDisplay the reaction diagram Show all sequences 3.2.1.167Ni-NTA column chromatography
Display the word mapDisplay the reaction diagram Show all sequences 3.2.1.167recombinant enzyme
Display the word mapDisplay the reaction diagram Show all sequences 3.2.1.167recombinant His-tagged enzyme from Escherichia coli strain Rosetta 2 (DE3) by nickel affinity chromatography and dialysis, native enzyme 1800fold from cell-free extract by repeated anion exchange chromatography and gel filtration, followed by dialysis
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