EC Number   |
|---|
    3.2.1.141 | - |
| 3.2.1.141 | by Ni2+-NTA-agarose affinity chromatography |
| 3.2.1.141 | Ni2+-NTA-agarose column chromatography, gel filtration |
| 3.2.1.141 | partial |
| 3.2.1.141 | Q-Sepharose anion exchange column chromatography |
| 3.2.1.141 | recombinant |
| 3.2.1.141 | recombinant His6-tagged enzyme mutant from Escherichia coli by nickel affinity chromatography, followed by anion exchange chromatography, and multiple dialysis steps |
| 3.2.1.141 | recombinant wild-type and mutant enzymes from Escherichia coli by anion exchange chromatography and gel filtration |
| 3.2.1.141 | wild-type enzyme purified by heat treatment, nucleic acid precipitation, and ion-exchange chromatography, 12fold. His-tagged MTHase partially purified from the cell-free extract directly by metal chelating chromatography, 14.7fold |
