EC Number   |
|---|
   3.2.1.136 | - |
| 3.2.1.136 | centrifuged supernatant concentrated with membrane filter, applied to Bio-Gel P100 gel filtration column, cellulolytic fractions pooled, lyophilized, dissolved, loaded onto hydroxylapatite column, last steps repeated, 4 degrees Celsius, purity monitored by SDS-PAGE and isoelectric focusing |
| 3.2.1.136 | His Trap column chromatography and Superose 6 gel filtration |
| 3.2.1.136 | MEP HyperCel column chromatography, SP Sepharose column chromatography, and XK 26/20 column chromatography |
| 3.2.1.136 | recombinant His-tagged full-length enzyme and isolated catalytic domain from Escherichia coli strain BL21Star (DE3) by immobilized metal affinity chromatography |
| 3.2.1.136 | recombinant His-tagged XynC from Escherichia coli strain BL21(DE3) by nickel affinity chromatography |
| 3.2.1.136 | shaking of wheat kernels with acetate buffer (25 mM, pH 5.0, 0.02% sodium azide) for 17 h, liquid phase dialyzed against 10 mM sodium acetate buffer, pH 5.0 at 7 degrees Celsius for 24 h, dialysate concentrated by freeze-drying and resuspension in water |
| 3.2.1.136 | to near homogeneity |
