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Search Purification (Commentary)

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EC Number Purification (Commentary) Reference
Display the word mapDisplay the reaction diagram Show all sequences 3.2.1.135- 393353, 646810, 646821, 750815, 752088
Display the word mapDisplay the reaction diagram Show all sequences 3.2.1.135ammonium sulfate precipitation, DEAE-Sepharose column chromatography, and Sephadex G-100 gel filtration 703502
Display the word mapDisplay the reaction diagram Show all sequences 3.2.1.135BD TALON spin column for 6x His-tag protein affinity purification, simultaneous SDS-PAGE zymogram with 1% soluble starch 697834
Display the word mapDisplay the reaction diagram Show all sequences 3.2.1.135gene purification by agarose gel electrophoresis, enzyme purification by size exclusion chromatography, elution via Superdex 200 10/300 GL column with 50 mM MOPS, pH7, 50 mM NaCl 696810
Display the word mapDisplay the reaction diagram Show all sequences 3.2.1.135Lactococcus lactis cytoplasmic fraction containing the alpha-amylase and pullulanase domain proteins, respectively, is purified by using Ni-nitrilotriacetic acid matrices, elution fractions analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis 695738
Display the word mapDisplay the reaction diagram Show all sequences 3.2.1.135recombinant enzyme 646820
Display the word mapDisplay the reaction diagram Show all sequences 3.2.1.135supernatant ultrafiltrated, DEAE Sepharose CLl-4B, Superose 6 699051
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