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Search Purification (Commentary)

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Results 1 - 7 of 7
EC Number Purification (Commentary)
Display the word mapDisplay the reaction diagram Show all sequences 3.2.1.135-
Display the word mapDisplay the reaction diagram Show all sequences 3.2.1.135ammonium sulfate precipitation, DEAE-Sepharose column chromatography, and Sephadex G-100 gel filtration
Display the word mapDisplay the reaction diagram Show all sequences 3.2.1.135BD TALON spin column for 6x His-tag protein affinity purification, simultaneous SDS-PAGE zymogram with 1% soluble starch
Display the word mapDisplay the reaction diagram Show all sequences 3.2.1.135gene purification by agarose gel electrophoresis, enzyme purification by size exclusion chromatography, elution via Superdex 200 10/300 GL column with 50 mM MOPS, pH7, 50 mM NaCl
Display the word mapDisplay the reaction diagram Show all sequences 3.2.1.135Lactococcus lactis cytoplasmic fraction containing the alpha-amylase and pullulanase domain proteins, respectively, is purified by using Ni-nitrilotriacetic acid matrices, elution fractions analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis
Display the word mapDisplay the reaction diagram Show all sequences 3.2.1.135recombinant enzyme
Display the word mapDisplay the reaction diagram Show all sequences 3.2.1.135supernatant ultrafiltrated, DEAE Sepharose CLl-4B, Superose 6
Results 1 - 7 of 7