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Search Purification (Commentary)

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EC Number Purification (Commentary)
Display the word mapDisplay the reaction diagram Show all sequences 3.12.1.B1-
Display the word mapDisplay the reaction diagram Show all sequences 3.12.1.B1expression in Escherichia coli results in the formation of inclusion bodies of the protein in an inactive form. The recombinant protein can be successfully activated after an in vitro refolding treatment. The enzyme requires exposure to an acidicenvironment during protein folding for activation
Display the word mapDisplay the reaction diagram Show all sequences 3.12.1.B1high salt concentrations are needed to release enzyme activity during cell disruption
Display the word mapDisplay the reaction diagram Show all sequences 3.12.1.B1partial
Display the word mapDisplay the reaction diagram Show all sequences 3.12.1.B1recombinant inactive wild-type and mutant enzymes refolded from Escherichia coli strain BL21 StarΒ™(DE3) inclusion bodies
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