EC Number |
---|
3.1.3.72 | - |
3.1.3.72 | in absence of Triton X-100, very little enzyme activity is extracted from seeds |
3.1.3.72 | partial purification, extraction with 0.1% Triton X-100 increases recovery by nearly 4fold |
3.1.3.72 | partially purified, 6.5fold, by gel filtration |
3.1.3.72 | recombinant non-glycosylated His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography, recombinant glycosylated nontagged enzyme from Pichia pastoris strain GS115(his4) by cation exchange chromatography |
3.1.3.72 | recovery is most complete when detergent or phospholipase C is included in extracting medium |
3.1.3.72 | selective precipitation by heat and ammonium sulfate followed by anion exchange and chromatofocusing chromatography and gel filtration, purified 3300fold, to homogeneity |