EC Number |
Reference |
---|
3.1.1.76 | - |
246542, 679540, 680871 |
3.1.1.76 | 12.6fold to homogeneity by DEAE ion exchange chromatography and gel filtration |
663666 |
3.1.1.76 | development of a rapid and easy immobilization method of the enzyme, the extracellular enzyme is tightly adsorbed onto a commercially available polypropylene support with high yield and specificity. The immobilized enzyme catalyzes almost the complete hydrolysis of poly(3-hydroxyoctanoic acid) polymer to (R)-3-hydroxyoctanoic acid monomers |
713824 |
3.1.1.76 | extracellualr enzyme 4.2fold to homogeneity |
729063 |
3.1.1.76 | extracellular enzyme by gel filtration and adsorption chromatography |
729031 |
3.1.1.76 | from strain M4-7 |
665995 |
3.1.1.76 | native extracellular enzyme by hydrophobic interaction chromatography, gel filtration, and ion exchange chromatography |
729420 |
3.1.1.76 | purified from culture medium |
246539 |
3.1.1.76 | recombinant extracellular enzyme by hydrophobic interaction chromatography, recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3)(pETBd1) by nickel affinity chromatography |
729030 |
3.1.1.76 | recombinant solubilized and refolded His-tagged enzyme from Escherichia coli strain M15 by nickel affinity chromatography. The refolded enzyme cannot be eluted with 1 M imidazole buffer, leading to an immobilized biocatalyst where PhaZ depolymerase is homogeneously distributed in the agarose support as shown by confocal scanning microscopy. Polyhydroxyoctanoate cannot be hydrolyzed by the immobilized biocatalyst, whereas the attached enzyme is active in the hydrolysis of 4-nitrophenyl alkanoate esters, which differed in their alkyl chain length |
715799 |