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Search Purification (Commentary)

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EC Number Purification (Commentary) Reference
Display the word mapDisplay the reaction diagram Show all sequences 3.1.1.53- 94420
Display the word mapDisplay the reaction diagram Show all sequences 3.1.1.53180fold enrichment 94408, 94410
Display the word mapDisplay the reaction diagram Show all sequences 3.1.1.532600fold enrichment 94408
Display the word mapDisplay the reaction diagram Show all sequences 3.1.1.533000fold 94417
Display the word mapDisplay the reaction diagram Show all sequences 3.1.1.53850fold enrichment 94408
Display the word mapDisplay the reaction diagram Show all sequences 3.1.1.53copurified with a 66000 Da esterase 94416
Display the word mapDisplay the reaction diagram Show all sequences 3.1.1.53from baculovirus infected insect Sf9 cells as GFP-fusion protein by ultracentrifugation, ion-exchange chromatography on Q-Sepharose FF column and dialysis 698131
Display the word mapDisplay the reaction diagram Show all sequences 3.1.1.53from fresh tissue by ultracentrifugation (dialysis of supernatant), followed by gel-filtration on Sephadex G-200 column, ion-exchange chromatography on Sephadex-DEAE-A-50 column, second ion-exchange chromatography on DEAE-Sephacel column, isoelectric focussing on ampholine of pH 3.5-9.5 and a saccharose gradient, and final dialysis, 866% purification compared to homogenate 698132
Display the word mapDisplay the reaction diagram Show all sequences 3.1.1.53from HEK293S cells by protein A-affinity chromatography followed by on-bead thrombin digestion 700987
Display the word mapDisplay the reaction diagram Show all sequences 3.1.1.53GSTrap column chromatography, and Superdex 200 gel filtration 749846
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