EC Number   |
|---|
   3.1.1.26 | - |
| 3.1.1.26 | 227fold |
| 3.1.1.26 | 263fold |
| 3.1.1.26 | 268fold |
| 3.1.1.26 | 520fold |
| 3.1.1.26 | ammonium sulfate precipitation, DEAE-Toyopearl 650 M colum chromatography, and HW-55F gel filtration |
| 3.1.1.26 | HiTrap Q HP column chromatography |
| 3.1.1.26 | native enzyme 163fold from Chlorella kessleri strain 11h to homogeneity, it is not possible to purify active enzyme in the absence of Triton X-100 or by using other detergents because the enzyme becomes inactive during the procedure. Recombinant His-tagged enzyme 15.5fold from Escherichia coli strain JM109 by 2 steps of nickel affinity chromatography |
| 3.1.1.26 | native enzyme from strain Stolk to homogeneity |
| 3.1.1.26 | Superdex 75 gel filtration and Macro-Prep DEAE column chromatography |
