EC Number |
---|
3.1.1.24 | 168fold, 6 step purification |
3.1.1.24 | 422fold |
3.1.1.24 | 94fold, 10 step purification |
3.1.1.24 | can not be separated from 4-carboxymuconolactone decarboxylase, EC 4.1.1.44, which catalyzes the preceding reaction. The gene pcaD, which encodes the 3-oxoadipate enol-lactone hydrolase is fused to a second gene pcaC, which encodes the decarboxylase, a protocatechuate catabolic gene cluster |
3.1.1.24 | ELH I and II, 290fold, and 550fold |
3.1.1.24 | Ni2+ affinity column chromatography and Superdex75 gel filtration |