2.7.8.B10 | enzyme Cls613a is tightly associated with the membrane and copurifies with its substrate phosphatidylglycerol and product cardiolipin, recombinant His-tagged enzyme Cls613a_53-483 from Escherichia coli strain Rosetta by nickel affinity chromatography,dialysis, and anion exchange chromatography, followed by gel filtration. Purification of sufficient amounts of Cls613a for determination of catalytic activity is not successful |
726689 |