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Search Purification (Commentary)

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EC Number Purification (Commentary) Reference
Show all pathways known for 2.7.8.B10Display the word mapDisplay the reaction diagram Show all sequences 2.7.8.B10- 726689, 727058, 727789
Show all pathways known for 2.7.8.B10Display the word mapDisplay the reaction diagram Show all sequences 2.7.8.B10enzyme Cls447a is tightly associated with the membrane and copurifies with its substrate phosphatidylglycerol and product cardiolipin, recombinant His-tagged enzyme Cls447a_52-482 from Escherichia coli strain Rosetta by nickel affinity chromatography,dialysis, and anion exchange chromatography, followed by gel filtration 726689
Show all pathways known for 2.7.8.B10Display the word mapDisplay the reaction diagram Show all sequences 2.7.8.B10enzyme Cls613a is tightly associated with the membrane and copurifies with its substrate phosphatidylglycerol and product cardiolipin, recombinant His-tagged enzyme Cls613a_53-483 from Escherichia coli strain Rosetta by nickel affinity chromatography,dialysis, and anion exchange chromatography, followed by gel filtration. Purification of sufficient amounts of Cls613a for determination of catalytic activity is not successful 726689
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