EC Number   |
|---|
    2.7.7.60 | - |
| 2.7.7.60 | at 4°C, from bacterial lysate by Ni-NTA metal affinity chromatography (elution with 200 mM imidazole), dialysis, concentration by ultrafiltration, yield of 2.8 mg/l culture |
| 2.7.7.60 | metal-chelating Hitrap clumn preloaded with Ni2+, Q-Sepharose anion-exchange chromatography to remove previously added thrombin |
| 2.7.7.60 | Ni2+-nitrilotriacetic acid column |
| 2.7.7.60 | Q-Sepharose, Phenyl-Sepharose, Superdex HiLoad |
| 2.7.7.60 | recombinant C-terminally His6-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography, the His tag is removed by thrombin enzyme, and followed by anion exchange chromatography and gel filtration, to 99% homogeneity |
| 2.7.7.60 | recombinant enzyme using His-tag |
| 2.7.7.60 | recombinant GST-tagged enzyme from Escherichia coli strain DH5alpha by glutathione affinity chromatography, the GST tag is removed by Prescission Protease (PPase) enzyme, and followed by anion exchange chromatography and gel filtration, to 99% homogeneity |
| 2.7.7.60 | recombinant His-tagged enzyme from Escherichia coli by nickel affinity chromatography and gel filtration |
| 2.7.7.60 | recombinant His-tagged enzyme from Escherichia coli strain BL21 Codon Plus (DE3)-RIL by metal affinity chromatography and ultrafiltration |
