EC Number   |
|---|
    2.7.7.43 | - |
| 2.7.7.43 | affinity chromatography and gel filtration |
| 2.7.7.43 | anti-FLAG affinity bead chromatography |
| 2.7.7.43 | by column chromatography on Sephacryl S-200 (gel filtration), Yellow-86-Agarose (affinity) and Phenyl-Sepharose (hydrophobic affinity) |
| 2.7.7.43 | C-His6-tagged CSS is purified using a nickel-nitrilotriacetic acid-agarose (Ni2+-NTA-agarose) affinity column |
| 2.7.7.43 | Ni-NTA column chromatography |
| 2.7.7.43 | partial |
| 2.7.7.43 | recombinant proteins are expressed in Escherichia coli BL21DE3 cells and purified by affinity chromatography utilizing the StrepII-Tag, further purified on a Superdex 200 HR 10/30 columnn |
| 2.7.7.43 | wild-type enzyme and mutant enzymes |
