EC Number   |
|---|
    2.7.7.3 | - |
| 2.7.7.3 | Co2 + affinity column chromatography |
| 2.7.7.3 | copurification as hexamer with 0.5 equivalents of CoA tightly bound per PPAT monomer |
| 2.7.7.3 | His Trap column chromatography, and Superdex 200 gel filtration |
| 2.7.7.3 | Ni-nitrilotriacetic acid-agarose affinity chromatography, gel filtration |
| 2.7.7.3 | Ni-NTA agarose column chromatography and Superdex 200 gel filtration |
| 2.7.7.3 | partial |
| 2.7.7.3 | Q Sepharose XL resin column chromatography, Q Sepharose HP resin column chromatography, and Sepharose S-200 gel filtration |
| 2.7.7.3 | recombinant enzyme PPAT from Escherichia coli strain BL21(DE3) to over 95% purity |
| 2.7.7.3 | recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography, dialysis, and ultrafiltration |
