EC Number   |
|---|
   2.7.4.33 | - |
| 2.7.4.33 | by gel filtration, separation from polyphosphate kinase activity |
| 2.7.4.33 | native enzyme 1511fold from strain 210A by streptomycin sulfate precipitation, two steps of anion exchange chromatography, hydrophobic interaction chromatography, and gel filtration |
| 2.7.4.33 | native enzyme 20.1fold by anion exchange, hydrophobic interaction, and affinity chromatography |
| 2.7.4.33 | native enzyme 200fold by membrane fraction preparation and washing with detergent Triton X-100 and SB-12 |
| 2.7.4.33 | partial |
| 2.7.4.33 | recombinant His-tagged enzyme from Escherichia coli strain Rosetta (DE3)pLysS by affinity chromatography |
| 2.7.4.33 | recombinant pPAP2 dimer 22.1fold from Escherichia coli strain JM109 by ammonium sulfate fractionation, anion exchange and hydroxyapatite chromatography, and gel filtration |
