EC Number   |
|---|
   2.7.4.22 | - |
| 2.7.4.22 | ammonium sulfate precipitation, DEAE cellulose column chromatography, Sephadex G-200 gel filtration, and nickel metal chelate affinity chromatography |
| 2.7.4.22 | heating at 70°C, ammonium sulfate precipitation, and column chromatography on Dyematrex Gel Red A and the anion exchange materials DE52 and Q6. Minor amounts (around 2%) of a smaller 15 kDa protein, presumably an SsUMPK degradation product, co-purified with SsUMP. |
| 2.7.4.22 | immobilized metal-affinity chromatography (IMAC) on a nickel column, SDS-PAGE, further purified by FPLC on an anion-exchange column, greater than 99% purity |
| 2.7.4.22 | metal affinity column using the gravity flow procedure, purity determined by SDS-PAGE |
| 2.7.4.22 | Ni-NTA affinity chromatography and gel filtration |
| 2.7.4.22 | Ni-NTA column chromatography |
| 2.7.4.22 | of the recombinant protein |
| 2.7.4.22 | of the recombinant protein by affinity chromatography |
| 2.7.4.22 | of the recombinant protein by affinity chromatography, no difference in activity compared to the wild type |
