EC Number   |
|---|
   2.7.3.4 | - |
| 2.7.3.4 | affinity chromatogrphy, concentrated to 10 mg ml-1 by lyophilization and resolubilized in MilliQ water, the purity is verified by the presence of a single band on overloaded Coomassie Blue-stained SDS-PAGE |
| 2.7.3.4 | amylose resin column chromatography, gel filtration |
| 2.7.3.4 | recombinant enzyme coding region as maltose binding protein fusion protein from Escherichia coli strain TB1 by amylose affinity chromatography too homogeneity. Recombinant N-terminally GST-tagged enzyme D1 and D2 mutants from Escherichia coli strain BL21(DE3) pLysSby glutathione affinity chromatography, the GST-tag is cleaved off by thrombin treatment |
| 2.7.3.4 | recombinant N-terminal His6-PsPK protein from Escherichia coli strain Rosetta 2 (DE3) by nickel affinity chromatography, ultrafiltration, and gel filtration |
| 2.7.3.4 | recombinant protein is purified by affinity chromatography using amylose resin |
| 2.7.3.4 | recombinant wild-type and mutant enzymes from Escherichia coli strain BL21(DE3) by Blue Sepharose affinity and anion exchange chromatography, followed by ultrafiltration |
