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Search Purification (Commentary)

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EC Number Purification (Commentary)
Show all pathways known for 2.7.1.2Display the word mapDisplay the reaction diagram Show all sequences 2.7.1.2-
Show all pathways known for 2.7.1.2Display the word mapDisplay the reaction diagram Show all sequences 2.7.1.2His-tagged recombinant TcGlcK is expressed in the Escherichia coli BL21 strain and purified to homogeneity by nickel affinity chromatography
Show all pathways known for 2.7.1.2Display the word mapDisplay the reaction diagram Show all sequences 2.7.1.2large scale
Show all pathways known for 2.7.1.2Display the word mapDisplay the reaction diagram Show all sequences 2.7.1.2recombinant
Show all pathways known for 2.7.1.2Display the word mapDisplay the reaction diagram Show all sequences 2.7.1.2recombinant enzyme
Show all pathways known for 2.7.1.2Display the word mapDisplay the reaction diagram Show all sequences 2.7.1.2recombinant enzyme from Escherichia coli strain BL21(DE3) by heat treatment and Ni2+ chelating affinity chromatography
Show all pathways known for 2.7.1.2Display the word mapDisplay the reaction diagram Show all sequences 2.7.1.2recombinant His-tagged enzyme from strain BL21(DE3) by nickel affinity chromatography
Show all pathways known for 2.7.1.2Display the word mapDisplay the reaction diagram Show all sequences 2.7.1.2recombinant His-tagged wild-type and mutant enzymes from Escherichia coli strain RB791 by nickel affinity chromatography
Show all pathways known for 2.7.1.2Display the word mapDisplay the reaction diagram Show all sequences 2.7.1.2recombinant soluble His-tagged enzyme from Escherichia coli strain BL21(DE3) by ultracentrifugation, nickel affinity chromatography, and gel filtration to homogeneity
Show all pathways known for 2.7.1.2Display the word mapDisplay the reaction diagram Show all sequences 2.7.1.2using Ni-NTA chromatography
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