EC Number |
---|
2.7.1.175 | native enzyme by heat treatment at 50°C for 30 min, anion exchange chromatography, ammonium sulfate fractionation, hydrophobic interaction chromatography, ultra- and gel filtration, and another step of anion exchange chromatography |
2.7.1.175 | recombinant C-terminally His-tagged wild-type and mutant enzymes from Escherichia coli strain BL21(DE3) by nickel affinity and anion exchange chromatography, followed by gel filtration |
2.7.1.175 | recombinant enzyme from Escherichia coli by anion exchange chromatography and gel filtration |
2.7.1.175 | recombinant His-tagged enzyme from Mycobacterium smegmatis by nickel affinity chromatography |
2.7.1.175 | recombinant His-tagged enzyme from Streptomyces lividans strain 66 by nickel affinity chromatography |