EC Number |
---|
2.7.1.151 | ammonium sulfate, Con A-Sepharose, DEAE-Toyopearl, 5'AMP-Sepharose |
2.7.1.151 | ammonuium sulfate, DEAE-cellulose |
2.7.1.151 | native enzyme partially by nuclear fraction preparation |
2.7.1.151 | recombinant enzyme |
2.7.1.151 | recombinant enzyme from Escherichia coli strain BL21(DE3) to near homogeneity by ion exchange and phosphocellulose resin chromatography |
2.7.1.151 | recombinant enzyme from strain expression in strain 12S16cpep4D by nickel affinity chromatography |
2.7.1.151 | recombinant GST-fusion isozymes from Escherichia coli by glutathione affinity chromatography |
2.7.1.151 | recombinant His-tagged wild-type and mutant enzymes from Escherichia coli by nickel affinity and heparin affinity chromatography, followed by tag cleavage through TEV protease, another step of heparin affinity chromatography, and gel filtration |
2.7.1.151 | recombinant N-terminally His-tagged enzyme from Escherichia coli strain BL21(DE3) by cobalt affinity chromatography |
2.7.1.151 | recombinant wild-type enzyme and deletion mutant from Escherichia coli by ion exchange and phosphocellulose resin chromatography |