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Search Purification (Commentary)

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Results 1 - 9 of 9
EC Number Purification (Commentary) Reference
Show all pathways known for 2.7.1.148Display the word mapDisplay the reaction diagram Show all sequences 2.7.1.148- 672138
Show all pathways known for 2.7.1.148Display the word mapDisplay the reaction diagram Show all sequences 2.7.1.148Hi-trap chelating columns are used for the purification of recombinant 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol kinase 699633
Show all pathways known for 2.7.1.148Display the word mapDisplay the reaction diagram Show all sequences 2.7.1.148recombinant enzyme 349728, 722218
Show all pathways known for 2.7.1.148Display the word mapDisplay the reaction diagram Show all sequences 2.7.1.148recombinant GST-tagged enzyme from Escherichia coli strain DH5alpha by glutathione affinity chromatography, tag cleavage through Prescission protease, followed by anion exchange chromatography, and gel filtration 747328
Show all pathways known for 2.7.1.148Display the word mapDisplay the reaction diagram Show all sequences 2.7.1.148recombinant His-tagged enzyme from strain BL21(DE3) to homogeneity by nickel affinity and anion exchange chromatography 663207
Show all pathways known for 2.7.1.148Display the word mapDisplay the reaction diagram Show all sequences 2.7.1.148recombinant His6-tagged enzyme by nickel affinity chromatography 660700
Show all pathways known for 2.7.1.148Display the word mapDisplay the reaction diagram Show all sequences 2.7.1.148recombinant His6-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography, tag cleavage through thrombin, followed by anion exchange chromatography, and gel filtration 747328
Show all pathways known for 2.7.1.148Display the word mapDisplay the reaction diagram Show all sequences 2.7.1.148recombinant selenomethionine-labeled enzyme from Escherichia coli strain B834(DE3) by ammonium sulfate fractionation, hydrophobic interaction and heparin affinity chromatography, gel filtration, and ultrafiltration 662149
Show all pathways known for 2.7.1.148Display the word mapDisplay the reaction diagram Show all sequences 2.7.1.148The gene is preceded by a His6 tag to enable purification of the recombinant protein via metal-chelating affinity chromatography. The polyhistidine tag is removed by thrombin-mediated proteolysis, followed by purification with anion-exchange chromatography. Purity of the sample is assessed by SDS-PAGE and MALDI-TOF MS. 686699
Results 1 - 9 of 9