EC Number   |
|---|
    2.7.1.147 | - |
| 2.7.1.147 | 600fold to homogeneity by ultracentrifugation, ion exchange chromatography, ammonium sulfate fractionation, hydrophobic interaction chromatography, gel filtration, hydroxyapatite chromatography, and 2 additional ion exchange chromatographic steps |
| 2.7.1.147 | recombinant enzyme from Escherichia coli strain BL21(DE3) by heat treatment at 90°C, ammonium sulfate fractionation, ion exchange chromatography, and gel filtration |
| 2.7.1.147 | recombinant enzyme from Escherichia coli strain DH5alpha by nickel affinity and ion exchange chromatography, to homogeneity |
| 2.7.1.147 | recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography, dialysis, and anion exchange chromatography |
| 2.7.1.147 | recombinant N-terminally His-tagged enzyme from Escherichia coli by heat treatment and nickel affinity chromatography |
| 2.7.1.147 | recombinant N-terminally His6-tagged enzyme from Escherichia coli strains BL21(DE3) and BL21-C41(DE3) by heat treatment at 50°C for 30 min, nickel affinity chromatography, dialysis, and anion exchange chromatography |
| 2.7.1.147 | recombinant N-terminally His6-tagged enzyme from Escherichia coli strains BL21(DE3) and BL21-C41(DE3) by nickel affinity chromatography, and dialysis |
| 2.7.1.147 | Resource Q column chromatography and Superdex S200 gel filtration |
