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Search Purification (Commentary)
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EC Number
Purification (Commentary)
2.6.1.9
-
2.6.1.9
220fold
2.6.1.9
C141-T1710, C141
2.6.1.9
from derepressed histidine auxotrophs, 95% pure
2.6.1.9
purified to homogeneity using nickel-nitrilotriacetic acid metal-affinity and gel filtration chromatography
2.6.1.9
recombinant protein
2.6.1.9
recombinant wild-type and selenomethionine-labeled enzyme from Escherichia coli strain BL21(DE3) and B834(DE3), respectively
2.6.1.9
use of omega-aminoalkylagaroses, 15fold purification in one step
2.6.1.9
wild-type and mutant enzymes
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