EC Number |
---|
2.6.1.52 | - |
2.6.1.52 | MalE/PdxC(SerC) fusion protein, affinity chromatography on an amylose resin, hydrolyzed in the presence of protease factor Xa |
2.6.1.52 | native enzyme from rat brain by ultracentrifugation at 220000 x g, followed by ammonium sulfate fractionation, hydropbobic interaction chromatography, anion exchange chromatography, and hydroxyapatite chromatography |
2.6.1.52 | recombinant |
2.6.1.52 | recombinant enzyme |
2.6.1.52 | recombinant enzyme 15.6fold from Escherichia coli strain BL21(DE3) by two different steps of anion exchange chromatography, ultrafiltration, and gel filtration |
2.6.1.52 | recombinant enzyme mutant K2G 5.5fold from Escherichia coli strain XL-1 Blue by anion exchange chromatography, ultrafiltration, and gel filtration |
2.6.1.52 | recombinant His-tagged wild-type and mutant enzymes from Escherichia coli strain BL21(DE3) by nickel affinity chromatography |
2.6.1.52 | recombinant His-tagged wild-type and mutant enzymes from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and gel filtration. The N-terminal 45 amino acid residues of wild-type EhPSAT protein can be truncated either at the time of purification or in vivo |
2.6.1.52 | recombinant His6-tagged from Escherichia coli strain BL21 Gold by nickel affinity chromatography, tag cleavage through TEV protease, dialysis, gel filtration and ultrafiltration |