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Search Purification (Commentary)

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Results 1 - 8 of 8
EC Number Purification (Commentary)
Display the word mapDisplay the reaction diagram Show all sequences 2.5.1.60-
Display the word mapDisplay the reaction diagram Show all sequences 2.5.1.60component B
Display the word mapDisplay the reaction diagram Show all sequences 2.5.1.60fresh plant material is frozen in liquid nitrogen, ground on ice with acid-washed sand in 100 mM Tris buffer, pH 7.8, containing 5 mM EGTA, 5 mM EDTA, 10 mM beta-mercaptoethanol, and 10% glycerol, lysate is centrifuged, dialyzed against 50 mM Tris buffer, pH 7.8, containing 10% glycerol, and 1 mM DTT
Display the word mapDisplay the reaction diagram Show all sequences 2.5.1.60nickel-Sepharose affinity chromatography
Display the word mapDisplay the reaction diagram Show all sequences 2.5.1.60recombinant enzyme
Display the word mapDisplay the reaction diagram Show all sequences 2.5.1.60recombinant His-tagged and c-Myc-tagged RGTA1 subunit from Saccharomyces cerevisiae strain YPH499 by affinity chromatography
Display the word mapDisplay the reaction diagram Show all sequences 2.5.1.60recombinant His-tagged and FLAG-tagged RGTB1 subunit from Saccharomyces cerevisiae strain YPH499 by affinity chromatography
Display the word mapDisplay the reaction diagram Show all sequences 2.5.1.60recombinant His-tagged and FLAG-tagged RGTB2 subunit from Saccharomyces cerevisiae strain YPH499 by affinity chromatography
Results 1 - 8 of 8