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Search Purification (Commentary)

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Results 1 - 6 of 6
EC Number Purification (Commentary) Reference
Show all pathways known for 2.5.1.56Display the word mapDisplay the reaction diagram Show all sequences 2.5.1.56- 637318, 637320, 637321
Show all pathways known for 2.5.1.56Display the word mapDisplay the reaction diagram Show all sequences 2.5.1.56cell harvesting by centrifugation, resuspension, lysing in high-pressure homogenizer, centrifugation, chromatographic procedures including ion-exchange and gel filtration steps, concentration 707514
Show all pathways known for 2.5.1.56Display the word mapDisplay the reaction diagram Show all sequences 2.5.1.56expression of the native neuB gene product enzyme in E. coli results in a product that is prone to proteolysis during purification so the protein is tagged with a hexa-histidine tag at its N-terminus and rapidly purified 637325
Show all pathways known for 2.5.1.56Display the word mapDisplay the reaction diagram Show all sequences 2.5.1.56Ni2+ affinity column chromatography 671716
Show all pathways known for 2.5.1.56Display the word mapDisplay the reaction diagram Show all sequences 2.5.1.56recombinant His-tagged enzyme from Escherichia coli strain Rosetta 2 by ultrafiltration, nickel affinity chromatography, and gel filtration 738339
Show all pathways known for 2.5.1.56Display the word mapDisplay the reaction diagram Show all sequences 2.5.1.56recombinant His6-tagged enzyme from Escherichia coli strain Rosetta 2 (DE3) pLysS by nickel affinity chromatography and gel filtration 737983
Results 1 - 6 of 6