EC Number   |
Reference |
|---|
    2.5.1.55 | - |
637393, 637394, 637395, 637413, 637416, 637420, 659323, 671293, 685148, 709520 |
| 2.5.1.55 | apoenzyme + 200 microM metal ion salt + 50 mM Tris-HCl buffer, pH 7.5 dialyzed against 50 mM ammonium acetate buffer, pH 7.5, 4°C |
710528 |
| 2.5.1.55 | enzyme purification followed by incubation with 50 microM Cd2+, Zn2+, or Cu2+ in 20 mM Tris-HCl buffer, pH 7.5, to substitute the metal ions Fe2+ or Co2+ |
707492 |
| 2.5.1.55 | one-step purification |
637405 |
| 2.5.1.55 | overnight dialysis against 50 mM ammonium acetate, pH 7.8, 4°C, reconstitution with substrates and products |
708132 |
| 2.5.1.55 | recombinant enzyme |
637422, 657841, 658666 |
| 2.5.1.55 | recombinant enzyme from wild-type strain AG701 and temperature-sensitive strain AG701i50 |
637419 |
| 2.5.1.55 | recombinant His-tagged enzyme from Escherichia coli strain BL21 (DE3) by nickel affinity chromatography and ultrafiltration |
739572 |
| 2.5.1.55 | recombinant His-tagged enzyme in Escherichia coli strain pTf16/BL21 by nickel affinity chromatography, dialysis, and ultrafiltration |
738157 |
| 2.5.1.55 | recombinant His6-tagged enzyme from Escherichia coli strain Rosetta2 (pLysS) DE3 by nickel affinity chromatography |
737364 |
