EC Number   |
|---|
    2.5.1.55 | - |
| 2.5.1.55 | apoenzyme + 200 microM metal ion salt + 50 mM Tris-HCl buffer, pH 7.5 dialyzed against 50 mM ammonium acetate buffer, pH 7.5, 4°C |
| 2.5.1.55 | enzyme purification followed by incubation with 50 microM Cd2+, Zn2+, or Cu2+ in 20 mM Tris-HCl buffer, pH 7.5, to substitute the metal ions Fe2+ or Co2+ |
| 2.5.1.55 | one-step purification |
| 2.5.1.55 | overnight dialysis against 50 mM ammonium acetate, pH 7.8, 4°C, reconstitution with substrates and products |
| 2.5.1.55 | recombinant enzyme |
| 2.5.1.55 | recombinant enzyme from wild-type strain AG701 and temperature-sensitive strain AG701i50 |
| 2.5.1.55 | recombinant His-tagged enzyme from Escherichia coli strain BL21 (DE3) by nickel affinity chromatography and ultrafiltration |
| 2.5.1.55 | recombinant His-tagged enzyme in Escherichia coli strain pTf16/BL21 by nickel affinity chromatography, dialysis, and ultrafiltration |
| 2.5.1.55 | recombinant His6-tagged enzyme from Escherichia coli strain Rosetta2 (pLysS) DE3 by nickel affinity chromatography |
