EC Number   |
Reference |
|---|
    2.5.1.32 | - |
2941, 637872, 637873, 637875, 637880, 637882 |
| 2.5.1.32 | partial |
637885 |
| 2.5.1.32 | Sephadex G-25 gel filtration and ammonium sulfate fractionation |
676639 |
| 2.5.1.32 | the purification protocol presents detailed instructions how to purify several milligrams of active enzyme from bacterial lysates, which includes initial recombinant enzyme enrichment through inclusion body purification, chaotropic unfolding, refolding in presence of detergents and purification through immobilized metal affinity chromatography |
759732 |
| 2.5.1.32 | to facilitate purification of the recombinant enzyme, the structural gene for Psase is modified by site-directed mutagenesis to incorporate a C-terminal Glu-Glu-Phe tripeptide to allow purification by immunoaffinity chromatography on an immobilized monoclonal anti-alpha-tubulin antibody YL1/2 column |
637884 |
