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Search Purification (Commentary)

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EC Number Purification (Commentary)
Show all pathways known for 2.5.1.22Display the word mapDisplay the reaction diagram Show all sequences 2.5.1.22-
Show all pathways known for 2.5.1.22Display the word mapDisplay the reaction diagram Show all sequences 2.5.1.22Cell lysate is loaded onto a HiTrap chelating column charged with Ni2+. The enzyme is eluted with an imidazole gradient (50-250 mM) at pH 8.0. The protein is loaded onto a Superdex 200 column equilibrated with 20 mM Tris-HCl and 150 mM NaCl. Thrombin is added to combined fractions containing SpmSyn to remove the His-tag. The protein is further purified to homogeneity by ion-exchange chromatography.
Show all pathways known for 2.5.1.22Display the word mapDisplay the reaction diagram Show all sequences 2.5.1.22His GraviTrap column chromatography, and gel filtration
Show all pathways known for 2.5.1.22Display the word mapDisplay the reaction diagram Show all sequences 2.5.1.22partial
Show all pathways known for 2.5.1.22Display the word mapDisplay the reaction diagram Show all sequences 2.5.1.22spermine-Sepharose affinity chromatography
Show all pathways known for 2.5.1.22Display the word mapDisplay the reaction diagram Show all sequences 2.5.1.22TALON affinity resin column chromatography
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