EC Number |
---|
2.4.2.14 | - |
2.4.2.14 | ammonium sulfate, heat treatment, calcium phosphate gel, DEAE-cellulose |
2.4.2.14 | ammonium sulfate, heat treatment, DEAE-Sepharose, Blue Dextran-Sepharose, hydroxylapatite |
2.4.2.14 | DEAE-cellulose, partial purification |
2.4.2.14 | from bacterial extracts by desalting on a Bio-Rad 10DG column in presence of 10 mM dithiothreitol, stored at -70°C |
2.4.2.14 | heat treatment, acid precipitation, ammonium sulfate, ethanol, Sephadex G-150, DE-52 |
2.4.2.14 | hexa-His-tagged mutant I198V and its variant 1-04 by metal affinity chromatography in presence of 1 mM beta-mercaptoethanol and 10% (v/v) glycerol, elution with 250 mM imidazole followed by dialysis |
2.4.2.14 | Ni-affinity column chromatography |
2.4.2.14 | partial |
2.4.2.14 | partial, ATP-agarose affinity chromatography |