EC Number   |
|---|
   2.4.1.7 | - |
| 2.4.1.7 | 6 × His-tagged recombinant enzyme is purified by Ni-NTA affinity chromatography |
| 2.4.1.7 | less than 10% impurities |
| 2.4.1.7 | mutant enzymes D295N and D295E |
| 2.4.1.7 | native enzyme 10fold, partially by gel filtration |
| 2.4.1.7 | partial |
| 2.4.1.7 | purification of thermostable mutant T47S/S62P/Y77H/V128L/K140M/Q144R/N155S/D249G by heating at 65°C for 20 min with 20% sucrose. The purified enzyme can be directly employed for the production of amylose with further purification |
| 2.4.1.7 | purified using standard immobilised metal affinity chromatography, facilitated by the fused 6-histidine tag |
| 2.4.1.7 | recombinant enzyme from Escherichia coli |
| 2.4.1.7 | recombinant enzyme from Escherichia coli by anion exchange and hydrophobic interaction chromatography |
