EC Number |
Reference |
---|
2.4.1.12 | Co-NTA column chromatography |
758133 |
2.4.1.12 | cobalt affinity column chromatography |
758221 |
2.4.1.12 | HiTrap column chromatography and Superdex 200 gel filtration |
758039 |
2.4.1.12 | IRX3 ist tightly bound to the cell wall and can only be released using very harsh conditions. Triton X-100 and NP40 are most effective. No effect: Triton X-114, Tween 80, Brij 35, Brij 58, CHAPS, octyl beta-glucoside, octyl beta-thioglucopyranoside |
661462 |
2.4.1.12 | native oligomers partially from microsomes as fragments of the cellulose synthase complex by dual epitope tagging of the CESA7 protein, selection for CESA multimers, overview. Recombinant His- and STREP-tagged CESA4, CESA7, and CESA8, as well as double mutant irx3-1:STREP-CESA7-irx3-1:His/FLAG-CESA7 from Arabidopsis thaliana irx1-1, irx3-1, and irx5-2 mutant plants by nickel and streptavidin affinity chromatography |
704608 |
2.4.1.12 | Ni-agarose column chromatography |
757973 |
2.4.1.12 | Ni-NTA agarose column chromatography |
755703 |
2.4.1.12 | recombinant His-tagged native and selenomethionine -labeled subunits BcsA and B from Escherichia coli strain C43 by nickel affinity chromatography and gel filtration |
736877 |