EC Number   |
|---|
   2.4.1.109 | conidia are incubated, filtered and washed with stop buffer (pH 7.0), ground in liquid nitrogen, centrifuged, supernatant centrifuged, pellet with crude membranes resuspended, resolved by SDS-PAGE, immunodetection of target protein AN5660 |
| 2.4.1.109 | gel purification of disruption mutants |
| 2.4.1.109 | glutathione Sepharose column chromatography |
| 2.4.1.109 | glutathione-Sepharose 4B bead chromatography |
| 2.4.1.109 | glutathione-Sepharose column chromatography |
| 2.4.1.109 | IgG-coupled magnetic bead chromatography |
| 2.4.1.109 | immunoaffinity chromatography, co-purification of PMT1 and PMT2 as a complex, no immuno-cross reactivity |
| 2.4.1.109 | isolation of microsome fraction: cells are homogenized in 10 mM Tris-HCl, pH 7.4, EDTA, sucrose, DTT, protease inhibitors, centrifugation, supernatant ultracentrifuged, precipitate used as microsomal fraction, solubilized in 20 mM Tris-HCl, pH 8.0, 2-mercaptoethanol, EDTA, n-octyl-beta-D-thioglucoside, centrifugation, supernatant used as solubilized microsome preparation, separation with SDS-PAGE and antibody staining |
| 2.4.1.109 | native enzyme partially |
| 2.4.1.109 | partial |
