2.4.1.101 | antibody purification from homogenized transfected Nicotiniana benthamiana leaves, resuspended in extraction buffer (PBS, pH 7.2, 1% Triton-X-100, protease inhibitor cocktail), centrifugation, supernatant incubated with rProtein A-Sepharose, elution by boiling Laemmli sample buffer, protein gel blot analysis with specific antibodies |