EC Number |
Reference |
---|
2.3.2.23 | Ni-NTA column chromatography and Superdex 75 gel filtration |
737253 |
2.3.2.23 | recombinant enzyme, N-terminally His-tagged, subcloned in Escherichia coli BL21(DE2), via pET28 vector, purified using Ni-NTA column and HiLoad 16/60 Superdex 75 gel filtration column, purity 95% after two steps |
723874 |
2.3.2.23 | recombinant GST- or His6-tagged enzyme UBE2R1 from Escherichia coli strain Rosetta (DE3) by glutathione or nickel affinity chromatography, respectively, followed by tag cleavage through TEV protease, and gel filtration |
759768 |
2.3.2.23 | recombinant His-tagged enzyme from Escherichia coli strain BL21 (DE3) Codon Plus by nickel affinity chromatography |
759820 |
2.3.2.23 | recombinant His-tagged wild-type and mutant enzymes from Escherichia coli by nickel affinity chromatography, followed by tag cleavage by TEV protease, dialysis, and gel filtration |
760168 |
2.3.2.23 | recombinant His6-tagged enzyme UBE2Z from Escherichia coli strain BL21(DE3) by metal affinity chromatography, dialysis, tag cleavage through HRV 3C protease, followed by anion exchange chromatography and dialysis, gel filtration and ultrafiltration |
759478 |
2.3.2.23 | recombinant protein, Ni-NTA column |
725413 |