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Search Purification (Commentary)

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Results 1 - 7 of 7
EC Number Purification (Commentary) Reference
Display the word mapDisplay the reaction diagram Show all sequences 2.3.2.23Ni-NTA column chromatography and Superdex 75 gel filtration 737253
Display the word mapDisplay the reaction diagram Show all sequences 2.3.2.23recombinant enzyme, N-terminally His-tagged, subcloned in Escherichia coli BL21(DE2), via pET28 vector, purified using Ni-NTA column and HiLoad 16/60 Superdex 75 gel filtration column, purity 95% after two steps 723874
Display the word mapDisplay the reaction diagram Show all sequences 2.3.2.23recombinant GST- or His6-tagged enzyme UBE2R1 from Escherichia coli strain Rosetta (DE3) by glutathione or nickel affinity chromatography, respectively, followed by tag cleavage through TEV protease, and gel filtration 759768
Display the word mapDisplay the reaction diagram Show all sequences 2.3.2.23recombinant His-tagged enzyme from Escherichia coli strain BL21 (DE3) Codon Plus by nickel affinity chromatography 759820
Display the word mapDisplay the reaction diagram Show all sequences 2.3.2.23recombinant His-tagged wild-type and mutant enzymes from Escherichia coli by nickel affinity chromatography, followed by tag cleavage by TEV protease, dialysis, and gel filtration 760168
Display the word mapDisplay the reaction diagram Show all sequences 2.3.2.23recombinant His6-tagged enzyme UBE2Z from Escherichia coli strain BL21(DE3) by metal affinity chromatography, dialysis, tag cleavage through HRV 3C protease, followed by anion exchange chromatography and dialysis, gel filtration and ultrafiltration 759478
Display the word mapDisplay the reaction diagram Show all sequences 2.3.2.23recombinant protein, Ni-NTA column 725413
Results 1 - 7 of 7