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Search Purification (Commentary)

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Results 1 - 10 of 10
EC Number Purification (Commentary) Reference
Show all pathways known for 2.3.1.31Display the word mapDisplay the reaction diagram Show all sequences 2.3.1.31- 486779, 672001, 673497
Show all pathways known for 2.3.1.31Display the word mapDisplay the reaction diagram Show all sequences 2.3.1.31cells are centrifuged in 50 mM Tris, pH 8.0, sonicated, centrifuged, supernatant loaded to a Ni2+-nitrilotriacetic acid-agarose column, washed, and eluted, eland incubated with protease and dialyzed, reloaded onto a Ni2+-nitrilotriacetic acid-agarose column, dialyzed, loaded onto Superdex-200 size-exclusion fast protein liquid chromatography column with 25 mM HEPES-buffer, pH 7.5 687796
Show all pathways known for 2.3.1.31Display the word mapDisplay the reaction diagram Show all sequences 2.3.1.31partial 486780, 486781, 486782, 486786
Show all pathways known for 2.3.1.31Display the word mapDisplay the reaction diagram Show all sequences 2.3.1.31partial, mutant strain 6 486784
Show all pathways known for 2.3.1.31Display the word mapDisplay the reaction diagram Show all sequences 2.3.1.31recombinant from E. coli 486788
Show all pathways known for 2.3.1.31Display the word mapDisplay the reaction diagram Show all sequences 2.3.1.31recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity and anion excange chromatography, followed by gel electrophoresis, dialysis, and ultrafiltration 735393
Show all pathways known for 2.3.1.31Display the word mapDisplay the reaction diagram Show all sequences 2.3.1.31recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3)-T1 by nickel affinity chromatography and two different steps of gel filtration 755982
Show all pathways known for 2.3.1.31Display the word mapDisplay the reaction diagram Show all sequences 2.3.1.31recombinant N-terminally His6-tagged enzyme from Escherichia coli strain BL21(DE3)pLysS by nickel affinity chromatography, dialysis and ultrafiltration 736358
Show all pathways known for 2.3.1.31Display the word mapDisplay the reaction diagram Show all sequences 2.3.1.31using Ni-NTA chromatography 684480
Show all pathways known for 2.3.1.31Display the word mapDisplay the reaction diagram Show all sequences 2.3.1.31using Ni-NTA-chromatography 687796
Results 1 - 10 of 10